GM-CSF Rat anti-Mouse, Biotin, Clone: MP1-31G6, eBioscience™

Description

Description: The MP1-31G6 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF), a 14 kDa cytokine secreted by activated T cells and macrophages. Applications Reported: MP1-31G6 has been reported for use in detection of mouse GM-CSF by ELISA. Applications Tested: The MP1-31G6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) in combination with the purified MP1-22E9 (14-7331) antibody for capture and recombit mouse GM-CSF (14-8331) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. Filtration: 0.2 μm post-manufacturing filtered.

GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombit GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS).