missing translation for 'onlineSavingsMsg'
Få mere at vide
Få mere at vide
Invitrogen™ GeneArt™ Platinum™ Cas9 Nuclease (1 μg/μL)
Beskrivelse
Indeholder
1 vial GeneArt Platinum Cas9 Protein
Maximum efficiency, minimal off-target cleavage, the Cas9 RNP complex can act immediately after it enters the cell and is rapidly cleared from the cell, minimizing the chance for off-target cleavage events when compared to vector-based systems.
GeneArt Platinum Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli that can be used for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system.
The Cas9 RNP complex can act immediately once it enters the cell since there are no requirements for transcription or translation as with vector- or mRNA-based systems.
Additionally, the Cas9 RNP complex is rapidly cleared from the cell minimizing the chance of off-target cleavage when compared to other systems (Kim, et al. 2014). Incorporation of a nuclear localization signal (NLS) aids delivery to the nucleus, thus increasing the rate of genomic DNA cleavage. Use our GeneArt Precision gRNA Synthesis Kit or GeneArt CRISPR Strings to make your target specific gRNA.
GeneArt™ CRISPR Nuclease mRNA features include:
GeneArt Platinum Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli that can be used for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system.
The Cas9 RNP complex can act immediately once it enters the cell since there are no requirements for transcription or translation as with vector- or mRNA-based systems.
Additionally, the Cas9 RNP complex is rapidly cleared from the cell minimizing the chance of off-target cleavage when compared to other systems (Kim, et al. 2014). Incorporation of a nuclear localization signal (NLS) aids delivery to the nucleus, thus increasing the rate of genomic DNA cleavage. Use our GeneArt Precision gRNA Synthesis Kit or GeneArt CRISPR Strings to make your target specific gRNA.
GeneArt™ CRISPR Nuclease mRNA features include:
- Streamline cell engineering by eliminating transcription and translation in the cell
- Eliminate time consuming cloning steps
- Minimize off-target cleavage due to rapid clearance of the protein complex from the cell
Certificeringer
CoA
Anbefalet opbevaring
Store at -5 to -30°C
Tekniske data
Tekniske data
| Koncentration | 1 μg/μL |
| Indhold og opbevaring | 1 vial GeneArt Platinum Cas9 Protein. Store at -5 to -30°C |
| Oversigt | GeneArt™ Platinum™ Cas9 Nuclease, 25μg, 1μg/μL Concentration, –5 to –30°C Storage Temperature, DNA Replication and Repair Research Category, For Research Use Only, Dry Ice Shipping Condition |
| Produkttype | GeneArt™ Platinum™ Cas9 Nuclease |
| Mængde | 25 μL, 25 μg |
Produkttitel
Ved at klikke på Send, anerkender du, at du kan blive kontaktet af Fisher Scientific med hensyn til den feedback, du har givet i denne formular. Vi deler ikke dine oplysninger til andre formål. Alle angivne kontaktoplysninger skal også vedligeholdes i overensstemmelse med vores Privatlivspolitik.
Ser du en mulighed for forbedring?