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Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit (with competent cells)
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Mængde:
10 reactions
Pakningsstørrelse:
10 reaktioner
Beskrivelse
Indeholder
- CRISPR OFP Nuclease Vector, linearized
- 10X Oligonucleotide Annealing Buffer
- DNase/RNase-free Water
- 5X Ligation Buffer
- T4 DNA Ligase
- U6 Forward Sequencing Primer
- Control, double stranded oligonucleotide
- OneShot(TM) TOP10 Chemically Competent Cells
Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter.
GeneArt CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells.
The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. Competent cells included for added convenience and highest cloning efficiencies.
GeneArt™ CRISPR Nuclease Vector system offers:
GeneArt CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells.
The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. Competent cells included for added convenience and highest cloning efficiencies.
GeneArt™ CRISPR Nuclease Vector system offers:
- Easy-to-design genome engineering system
- Affordable, ready-to-use cloning vectors
- Enrichment for hard to transfect or difficult to modify cell
Certificeringer
CoA
Overholdelse
CoA
Anbefalet opbevaring
Store in freezer (-5 to -30°C)
Tekniske data
Tekniske data
| Kloningsmetode | Restriction Enzyme/MCS |
| Indhold og opbevaring | • CRISPR OFP Nuclease Vector, linearized • 10X Oligonucleotide Annealing Buffer • DNase/RNase-free Water • 5X Ligation Buffer • T4 DNA Ligase • U6 Forward Sequencing Primer • Control, double stranded oligonucleotide Store the above at -5 to -30°C. • OneShot™ TOP10 Chemically Competent Cells Store at -80°C. |
| Detektionsmetode | Primer-probe |
| Form | Liquid |
| Format | Kit |
| Reaktionshastighed | Fast |
| Teknik | CRISPR-Cas9 |
| Produktlinje | GeneArt |
| Produkttype | Crispr Nuclease Vector With ofP Reporter Kit |
| Promotor | U6, CMV |
| Vis mere |
For Research Use Only. Not for use in diagnostic procedures.
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