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Gibco™ Geltrex™ Fleksibel LDEV-fri reduktionsfaktor basementmembranmatrix

Artikelnummer. 30564946
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Artikelnummer. 30564946 Leverandør Gibco™ Leverandørnr. A4000046701

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Geltrex Flex LDEV-Free Reduced Growth Factor Basement Membrane Matrix er en opløselig basementmembranmatrix, der er renset for EHS-tumorer. Understøtter dyrkning af epitel-, endotel-, glatte muskelceller, angiogenese, neuritudvækst, dannelse af galde canaliculi assays med polariserede hepatocytter.

Gibco Geltrex Flex LDEV-fri reduktionsfaktor basementmembranmatrix er en opløselig form for basalmembran, der renses fra Engelbreth-Holm-Swarm (EHS) tumorceller. De vigtigste komponenter i Geltrex Flex-matrixen omfatter laminin, kollagen IV, entactin og heparansulfatproteoglykan.

Opløsningen, som danner gel ved 37 °C, kan bruges til fremme og vedligeholdelse af en differentieret fænotype i forskellige cellekulturer, herunder primære epitelceller, primære neuroner og glatte muskelceller. Geltrex Flexmatrix er blevet brugt i 3D- og 2D-cellekultur, herunder angiogenese-assays, neurit-udvækst-assays og galle-canaliculi-dannelsesassays i polariserede hepatocytter til ADME-applikationer.

Features of Geltrex Flex LDEV-Free Reduced Growth Factor Basement Membrane Matrix

  • Each lot tested for the absence of lactose dehydrogenase elevating virus (LDEV), mycoplasma, and a broad panel of viruses and microorganisms
  • Lots tested for endotoxin, maintenance of gel stability, and HUVEC tube formation
  • Growth factor–reduced to improve lot-to-lot consistency
  • Formulated without phenol red to minimize potential for estrogen-like effects
  • Provided in recyclable glass vials
  • 1-mL, 5-mL, and 10-mL sizes for maximum flexibility. Use only what you need. Reduce need for extensive aliquoting and unwanted freeze-thaws.

Gain reliability and consistency with hESC/iPSC-qualified and organoid-qualified Geltrex Flex matrix

For culturing hESCs or iPSCs, it is recommended to use Geltrex Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix. hESC-qualified Geltrex Flex matrix provides all the benefits of standard Geltrex Flex matrix, with added quality controls for each lot. Each lot is tested with iPSCs and H9 hESCs for at least five passages to help ensure reliability and consistency in hESC/iPSC studies.

For culturing tumoroids and other organoids, Geltrex Flex LDEV-Free Organoid-Qualified Reduced Growth Factor Basement Membrane Matrix is recommended. Organoid-qualified Geltrex Flex matrix provides all the benefits of standard Geltrex Flex matrix, with added quality controls for each lot. Each lot is tested and supports culture of mouse intestinal organoids, mouse liver ductal organoids, and mouse airway organoids, as well as expansion of mouse small intestinal organoids for more than five passages with typical organoid budding morphology.

TRUSTED_SUSTAINABILITY

Specifications

Celletype Variety of cultured cells, including HUVEC, primary epithelial cells, neurons, endothelial cells, smooth muscle cells, hepatocytes.
Klassifikation Reduced Growth Factor
Koncentration 9 to 13 mg/mL
Form Frozen
Produkttype Basement Membrane Matrix
Serum niveau Serum-free
Sterilitet Sterile
Uden tilsætningsstoffer No Phenol Red
Mængde 1 ml
Forsendelsestilstand Dry Ice
Endotoksin niveau ≤ 4.5 EU/mL
Renhed eller kvalitetsklasse Cell Culture Grade
Arter Mouse
Kultur Type Adherent Cell Culture, Mammalian Cell Culture, Neuronal Cell Culture
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Må kun benyttes til forskningsformål. Må ikke anvendes ved diagnostiske procedurer.

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