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STEMCELL Technologies G418

Artikelnummer. 17120002
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Mængde:
250 mg
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Artikelnummer. Mængde
17120002 250 mg
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Artikelnummer. 17120002 Leverandør STEMCELL Technologies Leverandørnr. 03812

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Beskrivelse

Antibiotic for selecting transfected mammalian cells

  • Antibiotic G418 is an aminoglycoside related to gentamicin and is toxic to bacteria, yeast, higher plants, mammalian cells, protozoans, and helminths
  • G418 binds to 80S ribosomes in prokaryotic and eukaryotic cells and inhibits protein synthesis, resulting in cell death
  • Resistance to the antibiotic is conferred by aminoglycoside-3-phosphotransferase
  • Vectors containing the G418 resistance gene (neo) are commonly used for transfecting eukaryotic cells, with selection of transfected cells achieved by culturing them in the presence of G418
  • Cells may exhibit variable resistance to the antibiotic and can take up to a week to die
  • The G418 antibiotic is suitable for use in cell culture for the selection and maintenance of G418-resistant clones when using ClonaCell™-TCS Medium (Catalog #03814), ClonaCell™-CHO CD Medium (Catalog #03815), ClonaCell™-CHO ACF Medium (Catalog #03816), ClonaCell™­CHO CD Liquid Medium (Catalog #03817), or other cell culture media

Tekniske data

CAS 108321-42-2
Mængde 250 mg
Produkttitel
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