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Foxp3 Rat, PE, Clone: PCH101, Staining Set, eBioscience™

Artikelnummer. 15590707
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Artikelnummer. 15590707 Leverandør Invitrogen Leverandørnr. 72577640

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Beskrivelse

Indeholder

  • Fixation/Permeabilization Concentrate (4X) (cat. 00-5123): 30mL, store at 2-8°C. Avoid agitation.
  • Fixation/Permeabilization Diluent (cat. 00-5223): 100mL, store at 2-8°C.
  • Permeabilization Buffer (10X) (cat. 00-8333): 100mL, store at 2-8°C. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1x working solution.
  • Normal Rat Serum (cat. 24-5555): 100 μL, store at 2-8°C.
  • Anti-Human Foxp3 PE (clone PCH101, cat. 12-4776): 25 tests, store at 2-8°C. Light-sensitive material.

Antibody Staining Kit

This Anti-Human Foxp3 Staining Set contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3+ cells. The PCH101 monoclonal antibody reacts with the amino terminus of human Foxp3, also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55kDa protein and a member of the forkhead/winged-helix family of transcription factors. It was identified as the gene responsible for the X-linked lymphoproliferative disease observed in scurfy (sf) mice and in the human disorder, X-linked autoimmunity-allergic dysregulation syndrome (XLAAD). Constitutive expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

The PCH101 antibody crossreacts with rhesus, chimpanzee, and cynomolgus Foxp3. PCH101 recognizes a different epitope of Foxp3 than clones 236A/E7 and 150D/E4.
TRUSTED_SUSTAINABILITY

Tekniske data

Antigen Foxp3
Applikationer Flow Cytometry
Klassifikation Monoclonal
Klon PCH101
Konjugeret PE
Gene Alias Forkhead Box P3, Scurfin, JM2
Værtsarter Rotte
Mængde Kit
Regulatorisk status RUO
Primær eller sekundær Primary
Målarter Chimpanzee, Cynomolgus, ADN, Macaque, Abe, Non-human Primate, Rhesus abe
Indhold og opbevaring Store at 2-8°C. Do not freeze. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle.
Isotype IgG2a κ
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