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Fisher BioReagents™ TaqDNA polymerase

Artikelnummer. p-105898
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Sammensætning:
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial
10X assaybuffer B: Tris-HCl 100 mM, pH 9 ved 25 °C, KCl 500 mM og MgCl 2 25 mM opløsning i et separat hætteglas
Mængde:
100 Units
25 × 100 Units
2500 Units
5 × 100 Units
5 × 2500 Units
5 × 500 Units
500 Units
5x 2500 enheder
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100 enheder
2500 enheder
500 enheder
Pakke med 5
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Product Code. 10458923

missing translation for 'mfr': Fisher BioReagents™ FB600030

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Description

Tillader op til 100 cyklusser af amplifikation uden tab af aktivitet

  • Licensed for use in PCR
  • Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E. coli host
  • Has a 5«→3« exonuclease activity
  • Available with two different reaction buffers
  • Buffer A contains an optimized final concentration of 15mM MgCl2 for most PCR amplifications
  • Buffer B does not contain MgCl2; MgCl2 is provided in a separate vial as a 25mM solution
  • Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™
  • Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl2) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl2 solution in a separate vial

Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above

TRUSTED_SUSTAINABILITY

Specifications

Koncentration 5 U/μL
Sammensætning 10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial
Indhold og opbevaring -20°C
Oversigt Taq DNA Polymerase
Form Liquid
Format Poly Tube
Mængde 5 × 500 Units
Til brug med (applikation) Standard PCR
Opbevaringsbuffer Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
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