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Cytiva First-Strand cDNA Synthesis Kit Product Code.: 10218334

Cytiva First-Strand cDNA Synthesis Kit

Product Code. 10218334 Shop All Cytiva Products
55 Reactions, 1 set
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Quantity:
55 Reactions
Unit Size:
1 set
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Product Code. 10218334

Brand: Cytiva 27926101

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Includes

Bulk first-strand cDNA reaction mixes (Cloned murine reverse transcriptase, RNase inhibitor, RNase/DNase-free BSA, dATP, dCTP, dGTP, and dTTP in aqueous buffer), mRNA standard, DTT solution, RNase-free water, pd(N)6 primer, and Not I-(dT)19 bifunctional primer

For synthesis of single-stranded cDNA from mRNA templates prior to amplification by PCR

  • Preassembled bulk reaction mixes save time and minimize potential for pipetting errors
  • Primers are not included in the preassembled reaction mixes, allowing use of a primer of choice
  • Two primers are included with the kit
    • pd(N)6 primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5'-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail
    • Not I-(dT)18 bifunctional primer can be used to prime selectively on mRNA having a poly(A) tail and can also be used as a PCR primer following first-strand synthesis
    • The Not I restriction site, located at the 5'-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage -15°C to -30°C
Description Designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.
For Use With (Application) RT-PCR
Includes Bulk first strand cDNA reaction mixes, mRNA standard, DTT solution, RNase-free Water, pd(N)6 primer and NotI-(dT)18 bifunctional primer
No. of Reactions 55
Product Type First-Strand cDNA Synthesis Kit
Quantity 55 Reactions
Product Identifier
  • First-Strand cDNA Synthesis Kit, 55 reactions
Hazard Statement
  • EUH210-Safety data sheet available on request.
Supplemental information
  • UNKNOWN ACUTE-3 per cent of the mixture consists of component(s) of unknown acute toxicity.
  • UNKNOWN AQUATIC-Contains 25.5% of components with unknown hazards to the aquatic environment.
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