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Beschreibung
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.
Highlights
- Efficient—high yields of up to 20 μg of high quality plasmid DNA
- Fast—procedure takes less than 14 minutes
- Convenient—no phenol/chloroform extraction or alcohol precipitation required
- Pure—purified DNA is immediately ready to use
Applications
Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
- FastDigest or conventional restriction digestion
- Automated fluorescent and radioactive sequencing
- PCR
- In vitro transcription
- Transformation
Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing.
Components
- Resuspension Solution
- Lysis Solution
- Neutralization Solution Wash Solution (concentrated)
- RNase A Solution
- Elution Buffer
- Spin Columns
- Collection Tubes
- Detailed Protocol
Related Products
- RNase A (Part No.: R1253)
- Elution Buffer for GeneJET Plasmid Miniprep Kit (Part No.: R1263)
- Lysis Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1223)
- Resuspension Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1213)
- Neutralization Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1233)
- Wash Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1243)
- Binding Buffer for GeneJET Plasmid Miniprep Kit(Part No.: R1331)
Spezifikation
Spezifikation
| Column Type | Spin Column |
| Format | Spin Column |
| Isolation Technology | Silica Column |
| Scale | Mini |
| Sample Type | Bacteria |
| For Use With (Application) | PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription |
| High-throughput Compatibility | Compatible (Vacuum manifold) |
| Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
| Product Line | GeneJET |
| Product Type | Plasmid MiniPrep Kit |
| Mehr anzeigen |
Sicherheit und Handhabung
- GENEJET PL MINIPREP 250PREP
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: Sodium lauryl sulfate, Sodium hydroxide
For Research Use Only. Not for use in diagnostic procedures.
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