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Beskrivelse
Konventionelle restriktionsendonukleaser er en stor samling af højkvalitets restriktionsenzymer, optimeret til at arbejde i en af bufferne i Five Buffer System. Derudover leveres den universelle Tango-buffer for bekvemmelighed ved dobbeltfordøjelser. Alle enzymerne udviser 100 % aktivitet under de anbefalede buffer- og reaktionsbetingelser. For at sikre ensartet ydeevne, Thermo Scientific restriktionsenzymreaktionsbuffere indeholder forblandet BSA, som øger stabiliteten af mange enzymer og binder kontaminanter, der kan være til stede i DNA-præparater.
| 5' | T ↓ | G | T | A | C | A | 3' | ||||
| 3' | A | C | A | T | G ↑ | T | 5' |
Thermo Scientific Bsp1407I (BsrGI) restriction enzyme recognizes T^GTACA sites and cuts best at 37°C in Tango buffer (isoschizomers: BsrGI, BstAUI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
Tekniske data
Tekniske data
| Koncentration | 10 U/μL |
| Methyleringsfølsomhed | Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive |
| Enzym | Bsp1407I (BsrGI) |
| Kompatibel buffer | 10X Buffer Tango |
| Følsom over for varmeinaktivering | Yes |
| Optimal reaktionstemperatur | 37°C |
| Type IIS RE | No |
| Mængde | 300 enheder |
| Produkttype | Restriction Enzyme |
| Forskningskategori | Traditional Cloning |
Må kun bruges til undersøgelsesformål. Må ikke anvendes ved diagnostiske procedurer.
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