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  6. Invitrogen™ F4/80 Monoclonal Antibody (Cl:A3-1)

Invitrogen™ F4/80 Monoclonal Antibody (Cl:A3-1)

Artikelnummer. 15275899
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15245899 500 μg 500µg
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Artikelnummer. 15275899 Leverandør Invitrogen™ Leverandørnr. MA516632
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Forventet forsendelse: 11-11-2026
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Beskrivelse

Rat Monoclonal Antibody

This product requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hrs. Citrate buffer (pH 6.0) is recommended for tissues fixed for more than 24 hrs. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
TRUSTED_SUSTAINABILITY

Tekniske data

Antigen F4/80
Applikationer Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Radioimmune Assays (RIA), Western Blot, Immunocytochemistry
Klassifikation Monoclonal
Klon Cl:A3-1
Koncentration 1 mg/mL
Konjugeret Unconjugated
Formulering PBS with 0.09% sodium azide
Gene ADGRE1
Genadgangsnr. Q61549
Gene Alias ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
Gensymboler ADGRE1
Værtsarter Rat
Immunogen Thioglycollate stimulated peritoneal macrophages from C57/BL mice.
Oprensningsmetode Protein G
Mængde 250 μg
Regulatorisk status RUO
Primær eller sekundær Primary
Gen-id (Entrez) 13733
Målarter Mouse
Indhold og opbevaring Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Produkttype Antibody
Form Liquid
Isotype IgG2b
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