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Invitrogen™ F(ab)-Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody
Beskrivelse
F(ab)-Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody can detect all subtypes of Mouse IgG (Mouse IgG1,2a,2b). It showed minimum to no cross-reactivity to various species immunoglobulins tested. This Fab fragment antibody is expressed in vitro and purified as a recombinant construct. It is also engineered to deliver enhanced stability and sensitivity. The advantages of recombinant antibodies include-better specificity, animal origin-free formulation, and lot-to-lot consistency. F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Tekniske data
Tekniske data
| Antigen | Mouse IgG (H+L) |
| Applikationer | ELISA, Western Blot, Immunocytochemistry |
| Klassifikation | Recombinant Superclonal |
| Koncentration | 1 mg/mL |
| Konjugeret | Unconjugated |
| Formulering | PBS with 0.09% sodium azide; pH 7.2-7.4 |
| Værtsarter | Goat |
| Immunogen | Purified Mouse IgG. |
| Oprensningsmetode | Affinity chromatography |
| Mængde | 250 μL |
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