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Thermo Scientific™ EquiPhi29™ DNA polymerase

Artikelnummer. 17360680
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Mængde:
1000 U
250 U
Pakningsstørrelse:
Pakke med 250
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Artikelnummer. Mængde unitSize
17360680 1000 U -
15973292 250 U Pakke med 250
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price
Regulatory: This product contains a substance listed in Annex XIV to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.
Artikelnummer. 17360680 Leverandør Thermo Scientific™ Leverandørnr. A39391

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1952.10 DKK valid until 2026-03-20
BEDSTE tilbudspris! Brug kampagnekoden "27968" for at få din tilbudspris.
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price
Regulatory: This product contains a substance listed in Annex XIV to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Beskrivelse

Thermo Scientific EquiPhi29 DNA Polymerase er en proprietær phi29 DNA polymerase mutant udviklet gennem in vitro proteinudvikling.

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution. This enzyme is significantly improved over phi29 DNA polymerase in protein thermostability, reaction speed, product yield, and amplification bias, while retaining all the benefits of the wild-type enzyme.

EquiPhi29 DNA Polymerase possesses strong strand displacement activity and 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.

Features of EquiPhi29 DNA Polymerase
• Highest processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized
• Low amplification bias
• Extremely high yields of amplified DNA, even from small amounts of template
• Highly accurate DNA synthesis in a short time 

Applications
EquiPhi29 DNA Polymerase may be used in a wide variety of applications including:
• Unbiased whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Protein-primed DNA amplification
• Cell-free cloning of lethal DNA
• In situ genotyping with padlock probes
• RNA-primed DNA amplification

Notes
1. The addition of pyrophosphatase to the reaction mixture with EquiPhi29 DNA Polymerase may further enhance DNA synthesis
2. Because of the enzyme's 3'→5' proofreading exonuclease, 3’-modified primers are highly recommended.
3. EquiPhi29 DNA Polymerase can amplify DNA in a wide range of temperatures (30–45°C), with an optimal reaction temperature of 42°C.

TRUSTED_SUSTAINABILITY

Tekniske data

Produkttype Stand-alone enzyme
Til brug med (applikation) MDA-WGA, RCA
Polymerase EquiPhi29 DNA Polymerase
Mængde 1000 U
Indhold og opbevaring

• EquiPhi29 DNA Polymerase (100 μL at 10 U/μL)
• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1.0 mL)
• 110 mM DTT (0.25 mL)

Store at –15 to –25°C.
Optimal reaktionstemperatur 42°C
Forsendelsestilstand Dry Ice
Koncentration 10 U/μL
Reaktionstid 2 hours

Må kun bruges til undersøgelsesformål. Må ikke anvendes ved diagnostiske procedurer.

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