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Invitrogen™ Reactive Oxygen Species (ROS) Fluorometric Assay Kit
Beskrivelse
The Reactive Oxygen Species (ROS) Fluorometric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ROS in fresh tissue and live cell samples. ROS are highly reactive molecules that play a crucial role in various physiological and pathological processes, including oxidative stress, inflammation, and cellular signaling.
The assay works by utilizing the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) to detect the presence of ROS. DCFH-DA is taken up by cells and is converted to dichlorofluorescein (DCFH) by intracellular esterase. DCFH is oxidized by ROS to the strong fluorescent DCF. The fluorescence of DCF (Ex/Em 502/525 nm) is directly proportional to the amount of ROS present in the sample.
Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results
Tekniske data
Tekniske data
| Indhold og opbevaring | Reagent 1: –20°C Reagent 2: 2–8°C |
| Detektionsmetode | Fluorescence |
| Prøvetype | Cell Culture, Live Tissue |
| Til brug med (udstyr) | Spectrophotometer, Microplate Reader, Flow Cytometer |
| Inkubationstid | 30-60 min. |
| Mængde | 96 tests |
| Forsendelsestilstand | Dry Ice, 2–8°C |
| Target Kit navngivet | Reactive Oxygen Species (ROS) |
| Samlet analysetid | 60 min. |
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