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DreamTaq Hot Start PCR Master Mix, 2X; water, nuclease-free
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.
Fidelity (vs. Taq): 1X
Better reaction outcomes:
- Higher yield of target amplicons from low template amounts.
- Increased reaction specificity due to reliable hot-start technology.
- Wider range of amplicon lengths - routine amplification of genomic DNA fragments up to 6kb.
Enhanced convenience:
- Room temperature reaction set-up.
- Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer.
- Simplified workflow and minimized pipetting steps with 2X master mix format.
Fidelity (vs. Taq): 1X
Anbefalet opbevaring
Store at -20C.
Tekniske data
Tekniske data
| Indhold og opbevaring | Contains: • 20 x 1.25 mL DreamTaq Hot Start PCR Master Mix (2X) • 20 x 1.25 mL Nuclease-free water Store at -5 to -30°C. |
| GC-Rich PCR-ydelse | Low |
| Polymerase | DreamTaq DNA Polymerase |
| Reaktionshastighed | Standard |
| Forsendelsestilstand | Wet or Dry Ice |
| Hot Start | Built-In Hot Start |
| Overhæng | 3'-A |
| Reaktionsformat | SuperMix or Master Mix |
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