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Beskrivelse
Indeholder
DreamTaq Hot Start DNA Polymerase; 10X DreamTaq Green Buffer
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.
Better reaction outcomes:
Fidelity (vs. Taq): 1X
Better reaction outcomes:
- Higher yield of target amplicons from low template amounts.
- Increased reaction specificity due to reliable hot-start technology.
- Wider range of amplicon lengths - routine amplification of genomic DNA fragments up to 6kb.
- Room temperature reaction set-up.
- Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer.
- Direct loading of PCR products on a gel with DreamTaq Green buffer.
Fidelity (vs. Taq): 1X
Anbefalet opbevaring
Store at -20C.
Tekniske data
Tekniske data
| Indhold og opbevaring | •20 x 100 μL DreamTaq Hot Start DNA Polymerase •40 x 1.25 mL 10X DreamTaq Green Buffer (includes 20 mM MgCl2) Store at -5 to -30°C. |
| GC-Rich PCR-ydelse | Low |
| Polymerase | DreamTaq DNA Polymerase |
| Reaktionshastighed | Standard |
| Mængde | 4 x 2500 U |
| Forsendelsestilstand | Wet or Dry Ice |
| Hot Start | Built-In Hot Start |
| Overhæng | 3'-A |
| Reaktionsformat | Separate Components |
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