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Invitrogen™ Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

Artikelnummer. 10571474
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Mængde:
500 μL
Pakningsstørrelse:
0.5ml
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10571474 500 μL 0.5ml
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Artikelnummer. 10571474 Leverandør Invitrogen™ Leverandørnr. 31458

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Donkey Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. This antibody has been successfully used in Western blot, and ICC applications. Antibody Specificity: This antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Restoration and Storage: Store product at 2-8°C until opened. Restore with 0.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at -70°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Country of Origin: USA.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Tekniske data

Antigen Rabbit IgG (H+L) Cross-Adsorbed
Applikationer Immunohistochemistry, Western Blot, Immunocytochemistry
Klassifikation Polyclonal
Koncentration 0.8 mg/mL
Konjugeret HRP
Formulering PBS with 15mg/mL BSA and no preservative; pH 7.6
Værtsarter Donkey
Oprensningsmetode Antigen affinity chromatography
Mængde 500 μL
Regulatorisk status RUO
Primær eller sekundær Secondary
Målarter Rabbit
Indhold og opbevaring 4°C
Produkttype Secondary Antibody
Form Lyophilized
Isotype IgG
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