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Invitrogen™ DKK4 Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHDKK4X5
Additional Details : Weight : 0.33820kg
Description
The Human DKK4 ELISA quantitates Hu DKK4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu DKK4. Principle of the method The Human DKK4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2.Specifications
| Q9UBT3 | |
| 10 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA Kit | |
| Plasma, Serum, Supernatant | |
| 5 x 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 27121 | |
| <12% | |
| Pre-coated 96 well plates, Standard, Assay Diluent Concentrate, Stop Solution, Wash Buffer, Biotinylated Detection Antibody, SAV-HRP, Chromogen, Adhesive Plate Covers | |
| 5 x 96 Tests | |
| Biomarker-Related Pathways | |
| 2°C to 8°C | |
| Human | |
| 4 hr. 45 min. |
| 13.72 pg/mL to 10,000 pg/mL | |
| 10 pg/mL to 10,000 pg/mL | |
| Biotin | |
| Other Proteins | |
| This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
| Human | |
| Colorimetric Microplate Reader | |
| DKK4 | |
| <10% | |
| HRP | |
| RUO | |
| Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L | |
| DKK-4 | |
| 1 hr. 20 min. |