Compbead anti-Rat Ig, κ/Negative Control (FBS) Compensation Particles Set, Clone: G16-510E3, BD

The BD CompBeads Compensation Particles Anti-Rat Ig, kappa Set contains polystyrene microparticles, which are used to optimizefluorescence compensation settings for multicolor flow cytometric analyses. The set provides two populations of microparticles, the Anti-Rat Ig, kappa particles, which bind any rat kappa light chain-bearing immunoglobulin, and the Negative Control particles that have no binding capacity. When mixed together with a fluorochrome-conjugated rat antibody, the BD CompBeads Compensation Particles provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or automatically using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra. Use of the BD CompBeads Compensation Particles is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy7, APC-Cy7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.

Flow Cytometry