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Molecular Probes™ ChromaTide™ Texas Red™-12-dUTP

ChromaTide™ Texas Red™-12-dUTP

Brand: Molecular Probes™ C7631

6260.50 DKK valid until 2025-08-24
Use promo code "24117" to get your promotional price.

Product Code. 10307122

6770.00 DKK / Each

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Description

Molecular Probes™ ChromaTide™ dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide™ dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide™ Labeled Nucleotides Specifications:
Ex/Em of dye: Texas Red™-12-dUTP (595/615 nm)

Length of alkynylamino linker: 12 atoms

Methods for Incorporating ChromaTide™ Nucleotides Into Probes

Nick translation

Random primer labeling

End-labeling with terminal deoxynucleotidyl transferase

Reverse transcription

PCR amplification

See Methods for Enzymatic Incorporation of ChromaTide™ dUTPs for specific guidelines for each of these methods.

Alexa Fluor™ and BODIPY™ Fluorescent Dyes Make Excellent Probes
Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY™ and Alexa Fluor™ dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY™ dyes helps ensure minimal spectral overlap. The Alexa Fluor™ dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

Long Linkers Improve Performance
The ChromaTide™ dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

For complete listing of our ChromaTide™ Reagents: Molecular Probes ChromaTide™ and aha labeled nucleotides—Table 8.5.
For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Specifications

Content And Storage	Store in freezer (-5 to -30°C) and protect from light.
Shipping Condition	Wet Ice
Label or Dye	Texas Red™
Product Line	ChromaTide™, Texas Red™

Labeling Method	Direct Labeling
Concentration	1 mM
Quantity	25 μL

Product Suggestions Documents

Documents

Product Information

Manuals

Methods for Enzymatic Incorporation of ChromaTide dUTPs Version: 05-18-2010 File size: 567.6 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2383809	Certificate TypeCertificate of Analysis	Date19/01/2022	Catalog NumberC7631
Lot Number2559168	Certificate TypeCertificate of Analysis	Date28/09/2022	Catalog NumberC7631
Lot Number2211198	Certificate TypeCertificate of Analysis	Date07/04/2020	Catalog NumberC7631
Lot Number2080278	Certificate TypeCertificate of Analysis	Date20/03/2019	Catalog NumberC7631
Lot Number2561644	Certificate TypeCertificate of Analysis	Date05/12/2022	Catalog NumberC7631

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For Research Use Only. Not for use in diagnostic procedures.

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