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Invitrogen™ CDK3/cyclin E1 Recombinant Human Protein
CDK3/CyclinE1 is a member of the cyclin-dependent protein kinase family. Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Brand: Invitrogen™ PV6298
Additional Details : Weight : 0.01000kg
Description
CDK3/CyclinE1 promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis. The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported.
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for simple use and optimized performance
- Multiple pack sizes available for convenient use
Specifications
| Kinase Assay | |
| 50mM Tris-HCl pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol | |
| 10μg | |
| CDK3: None CyclinE1: CCNE1, CCNE | |
| Yes |
| Target & Lead Identification & Validation|Kinase Biology|Kinase Proteins & Expression Reagents|Protein Biology|Enzyme & Protein Activity Assays|Kinase Activity|Protein Assays and Analysis|Industrial & Applied Science|Pharma & Biopharma | |
| 61.3/73.4kDa | |
| Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. | |
| Human | |
| GST Tag |