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Invitrogen™ Human CDK2/cyclin A1, GST Tag Recombinant Protein

Artikelnummer. 15408943
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Mængde:
1 mg
10 μg
100 μg
Pakningsstørrelse:
100µg
10µg
1mg
Artikelnummer. Mængde unitSize
15408943 10 μg 10µg
15418943 100 μg 100µg
15428943 1 mg 1mg
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Artikelnummer. 15408943

Brand: Invitrogen™ PV6289

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Beskrivelse

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human CDK2 and CyclinA1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Protein Form: Recombinant The gene accession number for the subunits (CDK2/Cyclin A1) are NP_001789.2 and NP_001104515.1.

CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P24941
Koncentration See Label
Til brug med (applikation) Kinase Assay
Formulering 50 mM tris HCl with 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 10 mM glutathione, 150 mM NaCl, 25% glycerol and no preservative; pH 7.5
Gen-id (Entrez) 1017
Molekylvægt (g/mol) 60.2-78.5 kDa
Navn Human CDK2/cyclin A1, GST Tag
Mængde 10 μg
Gene Alias A630093N05Rik; CDC28; CDC2A; cdc2-related protein kinase; CDK1; CDK2; CDKN2; Cell division protein kinase 2; cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33(CDK2); p33CDK2; p34 protein kinase
Fælles navn CDK2
Protein længde 1-298
Konjugeret Unconjugated
Arter Human
Rekombinant Recombinant
Protein tag GST-tag
Indhold og opbevaring -80°C, Avoid Freeze/Thaw Cycles
Udtrykssystem Baculovirus
Form Liquid
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