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CD95 (APO-1/Fas), Super Bright 436, clone: 15A7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 62-0951-82

Additional Details : Weight : 0.01000kg

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Product Code. 15855503

  • 2612.00 DKK / 100µg


Estimated Shipment date
from Supplier 29-10-2018
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Description

Description

Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.Applications Reported: This 15A7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 105 to 10 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Specifications

Specifications

CD95 (APO-1/Fas)
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P25446
FAS
IgG1, kappa
100μg
4°C, store in dark, DO NOT FREEZE!
Primary
14102
Flow Cytometry
15A7
Super Bright 436
Fas
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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