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CD93 (AA4.1), Super Bright 436, clone: AA4.1, eBioscience™

Rat Monoclonal Antibody

Brand:  Invitrogen 62-5892-80

Additional Details : Weight : 0.01000kg

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Product Code. 15855533

  • 1137.00 DKK / 25µg


Estimated Shipment date
from Supplier 02-01-2019
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Description

Description

Description: The AA4.1 monoclonal antibody reacts with mouse C1qRp, an approximately 130-140kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen was originally identified as an antigen expressed on early stages of B cell development in bone marrow. Adult spleen and fetal liver also have detectable numbers of AA4.1 positive cells. In combination with other markers of hematopoietic progenitor cells such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the bone marrow Lymphoid-Committed Progenitors (CLP) can be segregated into more primitive and more differentiated subsets based on expression of AA4.1. Correlated expression of surface IgM (sIgM), CD23, and AA4.1 antigen has also been used to define three nonproliferative subpopulations of immature/transitional peripheral B cells designated: T1 (AA4.1+/CD23-/sIgMhi), T2 (AA4.1+/CD23+/sIgMhi), and T3 (AA4.1+/CD23+/sIgMlo). AA4.1 is also reported to be expressed in cytoplasmic vesicles in endothelial cells, megakaryoblasts, and platelets. It is reported that monoclonal antibodies AA4.1 and 493 recognize different epitopes of the same molecule.Applications Reported: This AA4.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AA4.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 105 to 10 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Specifications

Specifications

CD93 (AA4.1)
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
O89103
CD93
IgG2b, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
17064
Flow Cytometry
AA4.1
Super Bright 436
Cd93
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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