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CD45RA, Super Bright 645, clone: HI100, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 64-0458-42

Additional Details : Weight : 0.01000kg

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Product Code. 15855663

  • 2695.00 DKK / Pack of 100


Estimated Shipment date
from Supplier 02-01-2019
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Description

Description

Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI100 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^9 cells/test.Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Specifications

Specifications

CD45RA
Monoclonal
5μl/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P08575
PTPRC
IgG2b, kappa
100 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
5788
Flow Cytometry
HI100
Super Bright 645
PTPRC
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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