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CD45, Super Bright 645, clone: 30-F11, eBioscience™

Rat Monoclonal Antibody

Brand:  Invitrogen 64-0451-82

Additional Details : Weight : 0.01000kg

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Product Code. 15855653

  • 2665.00 DKK / 100µg


Estimated Shipment date
from Supplier 05-11-2018
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Description

Description

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Specifications

Specifications

CD45
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P06800
Leukocyte Common Antigen, LCA, Ly-5
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
30-F11
Super Bright 645
Ptprc
Liquid
PTPRC
IgG2b, kappa
100μg
4°C, store in dark, DO NOT FREEZE!
Primary
19264
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