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CD4, Super Bright 645, clone: RM4-5, eBioscience™

Rat Monoclonal Antibody

Brand:  Invitrogen 64-0042-82

Additional Details : Weight : 0.01000kg

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Product Code. 15855623

  • 2665.00 DKK / 100µg


Estimated Shipment date
from Supplier 05-11-2018
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Description

Description

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.Applications Reported: The RM4-5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Specifications

Specifications

CD4
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P06332
CD4
IgG2a, kappa
100μg
4°C, store in dark, DO NOT FREEZE!
Primary
12504
Flow Cytometry
RM4-5
Super Bright 645
Cd4
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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