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CD4, Super Bright 436, clone: GK1.5, eBioscience™

Rat Monoclonal Antibody

Brand:  Invitrogen 62-0041-80

Additional Details : Weight : 0.01000kg

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Product Code. 15865473

  • 1054.00 DKK / 25µg


Estimated Shipment date
from Supplier 30-10-2018
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Description

Description

Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5. Applications Reported: This GK1.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Specifications

Specifications

CD4
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P06332
CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
GK1.5
Super Bright 436
Cd4
Liquid
CD4
IgG2b, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
12504
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