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CD3 Monoclonal Antibody (UCHT1), Super Bright™ 436, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
1188.00 DKK - 2895.00 DKK
Specifications
Antigen | CD3 |
---|---|
Clone | UCHT1 |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15855473
|
Affymetrix eBioscience
62-0038-42 |
100 Tests |
2895.00 DKK
Pack of 100 |
In Stock Log in to see stock. |
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15845473
|
Affymetrix eBioscience
62-0038-41 |
25 Tests |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: The UCHT1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions.
CD3 Monoclonal antibody specifically detects CD3 in Human samples. It is validated for Flow Cytometry.Specifications
CD3 | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
UCHT1 | |
Flow Cytometry | |
Super Bright 436 | |
Mouse | |
Human | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
CD3E |
For Research Use Only