missing translation for 'onlineSavingsMsg'
Learn More

CD274 (PD-L1, B7-H1), APC-eFluor™ 780, clone: MIH1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 47-5983-41

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15862674

  • 962.00 DKK / Pack of 25


Estimated Shipment date
from Supplier 27-12-2018
Add to basket

Description

Description

Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.Applications Reported: This MIH1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MIH1 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5μL (0.5μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluorâ„¢ 780 emits at 780nm and is excited with the Red laser (633nm). Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647nm; Emission: 780nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD274 (PD-L1, B7-H1)
Monoclonal
5μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9NZQ7
PDL1, B7H1
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
MIH1
APC-eFluor 780
CD274
Liquid
CD274
IgG1, kappa
25 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
29126
Videos