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CD25, Super Bright 702, clone: BC96, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 67-0259-42

Additional Details : Weight : 0.01000kg

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Product Code. 15855723

  • 2346.00 DKK / Pack of 100


Estimated Shipment date
from Supplier 03-12-2018
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Description

Description

Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.5μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405nm) and emits at 702nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405nm; Emission: 702nm; Laser: Violet Laser
Specifications

Specifications

CD25
Monoclonal
5μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P01589
IL2RA
IgG1, kappa
100 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
3559
Flow Cytometry
BC96
Super Bright 702
IL2RA
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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