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BB700 Mouse Anti-Human CD25, Clone M-A251 (RUO)
Mouse monoclonal antibody that specifically binds to the 55 kDa type I transmembrane glycoprotein known as low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα).
Brand: BD Biosciences 566447
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Beskrivelse
- The M-A251 monoclonal antibody specifically binds to the 55 kDa type I transmembrane glycoprotein known as low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα).
- CD25 is expressed on regulatory T cells, activated lymphocytes (T and B), and monocytes.
- It associates with the IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form the high-affinity IL-2R complex.
- CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation.
- Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses.
- The antibody was conjugated to BD Horizon BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes.
- It is a polymer-based tandem dye developed exclusively by BD Biosciences.
- With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg., 695/40-nm filter).
- This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.
Tekniske data
| CD25 (IL-2 Receptor α) | |
| Monoclonal | |
| Brilliant Blue 700 (BB700) | |
| 100 Tests | |
| Secondary | |
| Human, Baboon, Cynomolgus, Rhesus Monkey | |
| IgG1 κ |
| Flow Cytometry | |
| M-A251 | |
| Mouse | |
| RUO | |
| CD25 (IL-2 Receptor α) | |
| Refrigerate (2 to 8°C) |
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