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CD223 (LAG-3), Super Bright 436, clone: 3DS223H, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 62-2239-41

Additional Details : Weight : 0.01000kg

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Product Code. 15855523

  • 1056.00 DKK / Pack of 25


Estimated Shipment date
from Supplier 30-10-2018
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Description

Description

Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).This 3DS223H antibody will recognize a formaldehyde-fixed epitope.Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3DS223H antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Specifications

Specifications

CD223 (LAG-3)
Monoclonal
5μl/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P18627
Lymphocyte Activation Gene-3
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
3DS223H
Super Bright 436
LAG3
Liquid
LAG3
IgG1, kappa
25 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
3902
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