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CD16, Super Bright 436, clone: eBioCB16 (CB16), eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 62-0168-42

Additional Details : Weight : 0.01000kg

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Product Code. 15865483

  • 2138.00 DKK / Pack of 100


Estimated Shipment date
from Supplier 02-01-2019
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Description

Description

Description: The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.Applications Reported: This 0168 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 0168 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5μL (0.06μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 436 can be excited with the violet laser line (405nm) and emits at 436nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405nm; Emission: 436nm; Laser: Violet Laser
Specifications

Specifications

CD16
Monoclonal
5μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
O75015, P08637
CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B;
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
eBioCB16 (CB16)
Super Bright 436
FCGR3A
Liquid
FCGR3A, FCGR3B
IgG1, kappa
100 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
2214, 2215
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