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CD140a (PDGFRA), Super Bright 436, clone: APA5, eBioscience™

Rat Monoclonal Antibody

Brand:  Invitrogen 62-1401-80

Additional Details : Weight : 0.01000kg

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Product Code. 15855513

  • 1054.00 DKK / 25µg


Estimated Shipment date
from Supplier 03-01-2019
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Description

Description

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 1.0μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^9 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Specifications

Specifications

CD140a (PDGFRA)
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P26618
PDGFRA
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
APA5
Super Bright 436
Pdgfra
Liquid
PDGFRA
IgG2a, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
18595
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