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CD137 Ligand (4-1BB Ligand), PerCP-eFluor™ 710, clone: 4H3, eBioscience™
Mouse Monoclonal Antibody
Brand: Invitrogen 46-5906-42
Additional Details : Weight : 0.01000kg
Description
This 4H3 monoclonal antibody recognizes human CD137 ligand, also known as 4-1BB ligand, TNFSF9, or CDw137L. Applications Reported: This 4H3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H3 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5μL (0.06μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluorâ„¢ 710 emits at 710nm and is excited with the blue laser (488nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488nm; Emission: 710nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
CD137 Ligand (4-1BB Ligand) | |
Monoclonal | |
5μL/Test | |
0.2%PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P41273 | |
41BBL, TNFRSF9, 4-1BBL, TNFSF9, CDw137L | |
Mouse | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Human |
Flow Cytometry | |
4H3 | |
PerCP-eFluor 710 | |
TNFSF9 | |
Liquid | |
TNFSF9 | |
IgG1, kappa | |
100 Tests | |
4°C, store in dark, DO NOT FREEZE! | |
Primary | |
8744 |