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CD137 Ligand (4-1BB Ligand), PerCP-eFluor™ 710, clone: 4H3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 46-5906-42

Additional Details : Weight : 0.01000kg

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Product Code. 15855443

  • 2346.00 DKK / Pack of 100


Estimated Shipment date
from Supplier 05-11-2018
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Description

Description

This 4H3 monoclonal antibody recognizes human CD137 ligand, also known as 4-1BB ligand, TNFSF9, or CDw137L. Applications Reported: This 4H3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H3 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5μL (0.06μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluorâ„¢ 710 emits at 710nm and is excited with the blue laser (488nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488nm; Emission: 710nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD137 Ligand (4-1BB Ligand)
Monoclonal
5μL/Test
0.2%PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P41273
41BBL, TNFRSF9, 4-1BBL, TNFSF9, CDw137L
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
4H3
PerCP-eFluor 710
TNFSF9
Liquid
TNFSF9
IgG1, kappa
100 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
8744
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