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CD11c, Super Bright 600, clone: 3.9, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 63-0116-42

Additional Details : Weight : 0.01000kg

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Product Code. 15855553

  • 2695.00 DKK / Pack of 100


Estimated Shipment date
from Supplier 30-10-2018
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Description

Description

Description: The 3.9 monoclonal antibody reacts with human CD11c, the 150kDa integrin alpha X chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.Applications Reported: This 3.9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.5μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 105 to 10 cells/test. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Specifications

Specifications

CD11c
Monoclonal
5μl/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P20702
ITGAX
IgG1, kappa
100 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
3687
Flow Cytometry
3.9
Super Bright 600
ITGAX
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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