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CD117 (c-Kit), Super Bright 600, clone: 2B8, eBioscience™

Rat Monoclonal Antibody

Brand:  Invitrogen 63-1171-80

Additional Details : Weight : 0.01000kg

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Product Code. 15855583

  • 1220.00 DKK / 25µg


Estimated Shipment date
from Supplier 27-12-2018
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Description

Description

Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.The 2B8 monoclonal antibody crossreacts with pig.Applications Reported: This 2B8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.06μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 105 to 10 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Specifications

Specifications

CD117 (c-Kit)
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P05532
KIT
IgG2b, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
16590, 396810
Flow Cytometry
2B8
Super Bright 600
Kit
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse, Porcine
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