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CD117 (c-Kit) Rat anti-Mouse, Porcine, Super Bright 600, Clone: 2B8, eBioscience™

Rat Monoclonal Antibody

Brand:  Invitrogen 63-1171-80

Additional Details : Weight : 0.01000kg

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Product Code. 15855583

  • 1220.00 DKK / 25µg


Estimated Shipment date
from Supplier 05-06-2019
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Description

Description

Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1 and 2 signaling pathway.
Specifications

Specifications

CD117 (c-Kit)
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P05532, Q2HWD6
KIT
IgG2b, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16590, 396810
Flow Cytometry
2B8
Super Bright 600
Kit
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse, Porcine
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