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CD105 (Endoglin) Monoclonal Antibody (MJ7/18), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1051-82
Additional Details : Weight : 0.01000kg
Description
Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin. This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis. Applications Reported: The MJ7/18 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. Applications Tested: The MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells or mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.Specifications
CD105 (Endoglin) | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Q63961 | |
ENG | |
Affinity chromatography | |
RUO | |
13805 | |
4° C | |
Liquid |
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot | |
MJ7/18 | |
Unconjugated | |
ENG | |
AI528660; AI662476; CD105; CD105 antigen; cell surface MJ7/18 antigen; Edg; END; Endo; Endoglin; endoglin (Osler-Rendu-Weber syndrome 1); Eng; HHT1; ORW; ORW1; S-endoglin; transmembrane glycoprotein | |
Rat | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.