Thermo Scientific™ CaptureSelect™ Factor X Affinity Matrix

Description

CaptureSelect Factor X Affinity Matrix was specifically designed for the purification of human Factor X from plasma and recombinant sources.

Features of the CaptureSelect Factor X Affinity Matrix include:

• Highly selective for human Factor X with no affinity for other human plasma proteins
• Binds both Factor X and Factor Xa in the presence of calcium
• Enables efficient purification of Factor X with high yields and retained activity
• Mild elution at neutral pH using EDTA
• Offers excellent scalability
• Non-animal-derived

Coagulation Factor X is a vitamin K-dependent, liver-produced serine protease that serves as the first enzyme in the coagulation cascade to form fibrin. It is a two-chain glycoprotein with a molecular weight of approximately 59 kDa. Factor X normally circulates in the plasma as an inactive molecule with activation of Factor X involved in both the intrinsic and extrinsic coagulation pathways.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

• Matrix: agarose-based
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect Factor X affinity ligand
• Ligand coupling method: epoxide
• Binding capacity: >10 mg/mL resin
• Binding conditions: 50 mM Tris, 150 mM NaCl, 5 mM CaCl₂, pH 7
• Elution conditions: 50 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol