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BrdU Monoclonal Antibody (MoBU-1), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen B35128

2604.00 DKK valid until 2024-06-28
Use promo code "21649" to get your promotional price.



Additional Details : Weight : 0.50000kg

Product Code. 10000998

  • 3255.00 DKK / Each
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Description

Description

The MoBU-1 clone has a high specificity for BrdU, in addition to no cross-reactivity with EdU. This clone can also readily detect bromouridine (BrU) incorporated into RNA or BrdUTP incorporated into DNA strand breaks via the TUNEL assay. Samples labeled with the Alexa Fluor™ conjugates of MoBU-1 can be directly imaged by fluorescence microscopy or high-content imaging. The unconjugated form and the biotin conjugate of this clone require a secondary antibody or a streptavidin conjugate for detection, respectively. The MoBU-1 clone is also available for flow cytometry applications.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

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Shipping Condition: Wet ice

Specifications

Specifications

BrdU
Monoclonal
0.1 mg/mL
PBS with 5mM sodium azide; pH 7.2
Mouse
Affinity chromatography
RUO
Chemical
Antibody
IgG
Immunocytochemistry, Immunohistochemistry
MoBU-1
Unconjugated
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
BrdU
350 μL
Primary
4° C
Liquid
Chemical
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For Research Use Only. Not for use in diagnostic procedures.