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BD Human IFN-γ ELISPOT Kit
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Mængde:
2 Plates
Beskrivelse
Indeholder
- Plates: 2 Pre-coated ELISPOT plates
- Biotinylated Detection Antibody
- Enzyme Conjugate (Streptavidin-HRP)
- Assay Diluent
- Wash Concentrate (20)
- PBS (10)
- AEC Substrate Buffer
- AEC Chromogen
- Certificate of Analysis, with lot-specific optimal dilution instructions
ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, 2,3 the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines.
- Cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis
Tekniske data
Tekniske data
| Antigen | IFN-gamma |
| Krydsreaktivitet | Human |
| Inkluderer | Antibody coated plate, Biotinylated Human IFN-g detection Antibody, Streptavidin HRP, wash concentrate, PBS (10X) concentrate, assay diluent, AEC Substrate Buffer, AEC chromogen |
| Mængde | 2 Plates |
| Indhold og opbevaring | 4°C |
| Til brug med (applikation) | Cloning, proliferation assays, flow cytometry or other methods of analysis |
| Produkttype | Human IFN-gamma ELISPOT kit |
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