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Proteintech™ AuthentiKine™ Human HGF ELISA Kit

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous HGF

Brand:  Proteintech™ KE00168-96T


Product Code. 16851576

  • 2611.34 DKK / 96 tests

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Description

Description

  • Recognizes human HGF
  • HGF most potent mitogen of mature hepatocytes in primary culture
  • Dericed from biologically inactive single chain precursor fo 728 amino acids localized on cell surface and extracellular matrix
  • HGF is pleiotropic cytokine which exerts variety of effects on several cells, being involved in regulating many biological processes

Process:

  • Antibody specific for HGF pre-coated onto microwells
  • HGF protein in samples captured by the coated antibody after incubation
  • After extensive washing, horseradish peroxidase (HRP)-conjugated detection antibody specific for HGF is added to detect captured HGH protein
  • For signal development, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

BC130284
0.26 ng/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
3.5 hr.
0.313 to 20 ng/mL
ISO 13485
Coated ELISA Kit
DAB
3082
Solid
<10%
-20°C to +4°C
P14210
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