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STEMCELL Technologies ArciTect™ tracrRNA Kit

Artikelnummer. 17199301
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Mængde:
10 nmol-sæt
20 nmol-sæt
5 nmol-sæt
Pakningsstørrelse:
1 sæt
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Artikelnummer. Mængde unitSize
17199301 10 nmol-sæt -
17109311 20 nmol-sæt -
17189301 5 nmol-sæt 1 sæt
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Artikelnummer. 17199301 Leverandør STEMCELL Technologies Leverandørnr. 76017

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Beskrivelse

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

  • ArciTect™ tracrRNA is a trans-activating CRISPR RNA (crRNA), one of two RNA components required to make a guide RNA (gRNA) for CRISPR-Cas9 genome editing
  • ArciTect™ tracrRNA contains a complementary sequence to the linker sequence in ArciTect™ crRNA, allowing fast and efficient gRNA duplex formation
  • It is recognized by all ArciTect™ Cas9 nucleases and is essential for ribonucleoprotein (RNP) complex formation
  • It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency
  • The ArciTect™ tracrRNA Kit includes tracrRNA as well as ArciTect™ Annealing Buffer (5X), which is required for forming a gRNA duplex between ArciTect™ tracrRNA and ArciTect™ crRNA
  • ArciTect™ crRNA is available by custom order for user-specified target sequences, in 2 nmol (Catalog #76010), 10 nmol (Catalog #76011), and 20 nmol (Catalog #76012) sizes
  • ArciTect™ Cas9 Nuclease is also available separately (Catalog #76001)

Tekniske data

Celletype Pluripotent Stem Cells
Mængde 10 nmol-sæt
Produkttitel
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