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MeltDoctor™ MeltDoctor™ HRM Master Mix

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

Brand:  Applied Biosystems™ 4409535

Additional Details : Weight : 0.10500kg

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Product Code. 10075704

  • 31180.00 DKK / Each

In stock, ready to ship: 2

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Requires : Template and primers

Description

Description

  • Low background fluorescence
  • High brightness in the presence of double-stranded DNA
  • Minimal temperature shift of DNA melting due to dye binding
  • Thermal stability to tolerate PCR cycling conditions
  • No inhibition of polymerase activity, resulting in high PCR efficiency
  • A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

Perform Mutation Scanning:
  • HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis
  • As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE)

Advantages of HRM for mutation scanning
  • Low reagent consumption, with little waste: HRM requires only a 20μL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
  • Simple, fast workflow: no additional instrumentation is required after PCR amplification;A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
  • Fast optimization: unlike DHPLC, thermal optimization is not required
  • Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction
    Quality Components
    • Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results
    • AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing non-specific product formation and enabling reactions to be set up at room temperature
    • MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

    Order Info

    Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice

Specifications

Specifications

7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
HRM (High Resolution Melting) Dye, SYTO™ 9
DNA (Genomic), cDNA
Fluorescent
Liquid
5000 reactions
AmpliTaq Gold 360 DNA Polymerase
HRM Master Mix
HRM (High Resolution Melting) Analysis, Mutation Scanning
SNPs (Unknown or Numerous), Epigenetic Targets
50mL
Wet Ice
Liquid
High
qPCR, RT-PCR
MeltDoctor™
Fast
20 mL, 50 mL
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For Research Use Only. Not for use in diagnostic procedures.