Goat anti-Rat IgM Heavy Chain Secondary Antibody, Alexa Fluor 594, Invitrogen™

Description

Goat anti-Rat IgM Heavy Chain Secondary Antibody, Alexa Fluor 594 conjugate, A-21213, from Invitrogen™. Species Reactivity: Rat; Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Host Species: Goat
Cross Reactivity: Rat
Isotype: IgG
Immunogen: Rat Mu immunonglobin
Formulation: 250μL purified antibody (2mg/mL) in PBS and 5mM sodium azide; pH 7.5
Goat anti-rat IgM antibodies have been purified by rat IgM-affinity chromatography and react with the mu chain of rat IgM, as determined by ELISA. The rat anti-IgM may also react with IgM from other species.
References:
Reference: Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos. (2008) Nat Protoc, Vol: 3, 5, 923-33, Lerou PH,Yabuuchi A,Huo H,Miller JD,Boyer LF,Schlaeger TM,Daley GQ; Pubmed ID: 18451800