OxPhos Blue Native WB Mouse anti-Bovine, Human, Mouse, Invitrogen™

Description

This Anti-OxPhos Complex Kit is a cocktail of monoclonal antibodies specific to human, bovine, mouse OxPhos Complex This antibody cocktail is validated for use in Western blotting. Mouse monoclonal [20C11B11B11] to (C-I) NDUFA9: Amount: 120 μg Wo

Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress. Blue Native polyacrylamide gel electrophoresis (BN-PAGE) is a simple and effective way to subfractionate mitochondrial proteins as intact complexes on a single gel (in one dimension). It can be used to detect altered assembly of these complexes arising from mutations in subunits, mutations in assembly factors, or mtDNA depletion. This type of analysis has been performed with biopsy samples, platelets and fibroblast cells from patients with suspected mitochondrial diseases.