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Beskrivelse
The C8.6 antibody reacts with human IL-12 p40 monomers, homodimers, and p70 heterodimers, but not p35 subunits. The immunogen used to generate the C8.6 hybridoma was the purified p40 subunit of human IL-12. The C8.6 antibody is a neutralizing antibody and has been reported to strongly inhibit three different biological activities of IL-12 (i.e., IFN- γinduction), mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity. C8.6 has been shown to immunoprecipitate IL-23.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
Tekniske data
| IL-12 (p40/p70) | |
| Monoclonal | |
| Brilliant Violet 421 | |
| Aqueous buffered solution containing BSA and ≤0.09% sodium azide | |
| Purified p40 Subunit of Human IL-12 | |
| 100 Tests | |
| Primary | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Flow Cytometry | |
| C8.6 | |
| IL12B; IL-12B; IL12p40; IL-12-40; CLMF; CLMF2; NKSF; NKSF2 | |
| Mouse | |
| Affinity Purified | |
| RUO | |
| Human | |
| IgG1 κ |
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