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STEMCELL Technologies Annexin V, APC

Artikelnummer. 17107561
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Mængde:
100 tests
25 tests
Pakningsstørrelse:
100 tests
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17117561 100 tests 100 tests
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Artikelnummer. 17107561 Leverandør STEMCELL Technologies Leverandørnr. 1000328

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Beskrivelse

A cellular protein for detection of apoptotic cells, PE-conjugated

  • Annexin V is a member of the annexin family of proteins that bind to membrane phospholipids in the presence of calcium
  • This dye has high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane
  • During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, exposing it to the external environment
  • Annexin V, a characteristic marker for early cell apoptosis, detects the translocation of PS to the external environment
  • Annexin V is used along with viability dyes such as 7-AAD (Catalog #75001) or Propidium Iodide (Catalog #75002)
  • The process of PS translocation occurs prior to the loss of membrane integrity
  • Therefore, as cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently, viability dyes pass into the cell
  • Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and the viability dye

Tekniske data

Mængde 25 tests
Til brug med (applikation) Flow Cytometry
Konjugeret APC (Allophycocyanin)
Produkttitel
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