missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ MessageAmp™ II aRNA Amplification Kit

Brand:  Invitrogen™ AM1751

Additional Details : Weight : 0.12000kg

 View more versions of this product

Product Code. 10056234

  • 17000.00 DKK / Each

In stock, ready to ship: 2

Please to purchase this item. Need a web account? Register with us today!


Includes: 60μL T7 Oligo(dT) Primer; 22μL ArrayScript™ Reverse Transcriptase; 22μL RNase Inhibitor; 42μL 10X First Strand Buffer; 170μL dNTP Mix; 210μL 10X Second Strand Buffer; 42μL DNA Polymerase; 22μL RNase H; 84μL T7 Enzyme Mix; 84μL T7 10X Reaction Buffer; 84μL each T7 ATP, CTP, GTP, and UTP; 40μL Second Round Primers; 10μL Control RNA 1mg/mL; 1.75mL Nuclease-free Water; 30mL Wash Buffer; 7mL cDNA Binding Buffer; 9mL aRNA Binding Buffer; 20 aRNA Filter Cartridges; 40 aRNA Collection Tubes; 20 cDNA Filter Cartridges and Tubes; 20 cDNA Elution Tubes; 10mL Nuclease-free Water

Description

Description

System overview
Expression profiling using gene arrays has become the most widely used method for assessing gene expression on a genome-wide/scale. The primary limitation of this technology is the requirement for large amounts of RNA for array hybridization. This hurdle is usually overcome by using T7 RNA polymerase-driven transcription of cDNA (obtained from total RNA samples) to amplify sufficient amounts of cRNA. This amplification method is the basis of the MessageAmp II aRNA Amplification Kit.

An improved kit for cDNA amplification
Included in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The use of ArrayScript RT in conjunction with the optimization of the second-strand cDNA synthesis reaction results in increased conversion of mRNA into full-length, double-stranded cDNA templates. In addition, since the in vitro transcription (IVT) uses MEGAscript™ technology, the IVT incubation times are shortened. These improvements in the MessageAmp II kit also carry through to two-round reactions, facilitating robust amplification of total RNA from samples as low as 100pg.

Accessory products
Bio-11-UTP (Cat. No. AM8450) and Bio-16-UTP (Cat. No. AM8451) can be used in MessageAmp aRNA amplification reactions for target labeling for GeneChip analysis. The Bio-UTPs are confirmed free of nonspecific endonuclease activity, exonuclease activity, and RNase activity, and are functionally tested using the MessageAmp II aRNA Amplification Kit.

  • Increased amplification: up to 5,000-fold
  • Greater sensitivity due to increased yields of full-length cDNA
  • GeneChip analysis with as little as 100ng input RNA
  • Efficient labeling produces high percent present calls and low 3'/5' ratios
  • Includes ArrayScript™ RT, a specifically engineered M-MLV reverse transcriptase
  • Optimized second strand cDNA synthesis reaction

Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Amplification

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

Specifications

No
Poly(A+) RNA, Total RNA
aRNA Amplification Kit
ArrayScript™
Ambion™, MessageAmp™
20 Reactions
Videos
SDS
Documents
Certificates